Photomicrographs of cultivated human retinal epithelial cells seeded on platelet gel versus controls.

Photomicrographs of cultivated hRPE cells seeded on PG3 versus controls. Note the
epithelioid morphology of the control cells on days 3 (A) and 7 (B), and an elongated morphology
of the PG3-treated cells with dendritiform extensions on day 3 (C). The PG3 treated cells
demonstrate the epithelioid morphology on day 7 (D). Both control (E, I, M) and PG3-treated cells
(G, K, O) are stained positively (green) for the fluorescein isothiocyanate (FITC)-conjugated
cytokeratin 8/18 antibody (E & G), FITC-conjugated RPE65 antibody (I & K), and FITCconjugated
PAX6 antibody (M & O). Note DAPI-stained corresponding hRPE cells nuclei (blue)
in images F, J, and N for controls, and in images H, L, and P for PG3-treated cells. (Overall
magnification in all images × 200).