Double immunofluorescence labeling of Syt IV and PRL. Nuclei were counterstained with DAPI. (A) Adenohypophysis of rats from treatment group E2. (B) Adenohypophysis of rats from treatment group E2+L-dopa. (C) Adenohypophysis of intact control animals. Scale bars: 10 μm. E2 – 17b-estradiol; PRL – prolactin; SYT IV – synaptotagmin IV; L-dopa – levodopa.