Molecular mechanism of miR-212-3p in AD-induced neuroinflammation. SP1 bounds to the BACE1 promoter region to activate BACE1 expression. miR-212-3p inhibited SP1 and then BACE1 to block the activation of the NLRP3/Caspase-1 signaling pathway, thus attenuating neuroinflammation in AD rats.FIGURE 6. BACE1 upregulation activated NLRP3/Caspase-1 signaling pathway to reverse the inhibitory role of miR-212-3p over-expression in neuronal pyroptosis. (A) BACE1 was detected through RT-qPCR after H19-7 cells were transfected with oe-NC and oe-BACE1; (B) cell viability was assessed through the MTT method after Aβ1-42-treated H19-7 cells were transfected miR-212-3p mimic and oe-BACE1; (C) NLRP3, cleaved Caspase-1, and GSDMD-N expressions were determined through Western blot analysis; (D-E) IL-1β and IL-18 levels were measured through Western blot analysis (D) and ELISA (E). Cell experiments were conducted 3 times. Data were formalized as mean ± SD. Data in Figures A were analyzed using T test, data in Figure B were analyzed using one-way ANOVA, and data in Figures C-E were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test.DCBAE

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